For experiments executed in U2OS A3B KO cells, WT A3B-eGFP or catalytic mutant A3B-E255A-eGFP was cotransfected with mCherry-RNaseH1 mut and cells expressing both of those constructs were being analyzed. For GFP sign of ectopically expressed A3B, samples were being analyzed which has a 488 nm diode laser and acceptable filter https://kategoriemarketing19764.daneblogger.com/22370581/details-fiction-and-site-directed-mutagenesis